Anti-Broad bean mottle virus [NbBBMV01]

Description:

Recombinant monoclonal antibody to Broad bean mottle virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display clone NbBBMV01.

Clone Name:

NbBBMV01

Published Application(s):

neutralize, ELISA

Published Species Reactivity:

Broad bean mottle virus (BBMV)

Alternative Name(s) of Target: BBMV
Immunogen: The original antibody was isolated from a large 'immune' library constructed from an immunized Arabic camel using phage display technology and by panning against Broad ban mottle virus (BBMV).

Specificity: This antibody recognizes and binds Broad bean mottle virus (BBMV). BBMV (Bromovirus, Bromoviridae) is one of a number of viruses which are seedborne in a range of temperate pulses and has been found in Africa, Asia, Europe and the Middle East. The virus is seed borne in Cicer arietinum (chickpea), Pisum sativum (field pea) and Vicia faba (faba bean, broad bean, tick bean).

Application Notes: In vitro pre-incubation the original antibody and their viral target inhibited the BBMV infectivity and arrested its spreading in inoculated Vicia faba plants. This was demonstrated by lack of symptoms of viral infection (15 days post mechanical inoculation), in plants pre-blocked with this nanobody. The original nanobody when transiently expressed in plants has virus neutralizing capability (PMID: 25648551).

Antibody first published in: Ghannam et al. Camelid nanobodies with high affinity for broad bean mottle virus: a possible promising tool to immunomodulate plant resistance against viruses. Plant Mol Biol. 2015 Mar;87(4-5):355-69. PMID:25648551
Note on publication: This paper describes the generation of camelid nanobodies against Broad bean mottle virus (BBMV).