United Kingdom (UK)
Recombinant monoclonal antibody to Cap protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb15.
UniProt Accession Number of Target Protein: O56129
Alternative Name(s) of Target: Capsid protein; Cap; ORF2; anti-PCV2-Nb15
Immunogen: The original antibody was generated by immunizing Bactrian camel (Camelus bactrianus) with recombinant PCV2-Cap protein, followed by antibody library construction and phage display-based selection.
Specificity: The antibody binds to PCV2 Cap protein. There was no cross-reaction when detecting positive sera against other porcine viruses. the M2 cytoplasmic domain interacts directly with mammalian host cell proteins (e.g., in the autophagy pathway) and plays an important role in regulating viral replication.
Application Notes: The ability of this antibody (VHH) to recognize PCV2 Cap protein was confirmed by indirect ELISA analysis. The antibody was used to develop a competitive ELISA for detecting PCV2 antibody levels in porcine serum samples (Mu et al, 2021; pmid:33526021).
Antibody first published in:
Mu et al. A nanobody-horseradish peroxidase fusion protein-based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2 J Nanobiotechnology. 2021 Feb 1;19(1):34. PMID:33526021
Note on publication:
The paper describes the generation and characterization of the antibody.