United Kingdom (UK)
Recombinant monoclonal antibody to CD104. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 58XB4.
UniProt Accession Number of Target Protein: P16144
Alternative Name(s) of Target: Integrin beta-4; ITGB4; GP150
Immunogen: The mouse version of this antibody was raised by immunizing a BALB/c mouse with the full length CD104 protein.
Specificity: This antibody is specific for human CD104. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.
Application Notes: To investigate the recruitment of plectin into hemidesmosomes by the α6β4 integrin, immunohistochemistry was performed on human MD-EBS keratinocytes using the mouse version of this antibody (Geerts et al, 1999; pmid:10525545). While showing that a subpopulation of cells in a hiPSC-derived corneal epithelial cell sheet is positive for ABCB5, flow cytometry was performed on human hiPSC-derived SEAMs using the mouse version of this antibody (Watanabe et al, 2021; pmid:34195566). The mouse version of this antibody was used in flow cytometry on differentiated human iPS cells to demonstrate the generation of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells using human induced pluripotent stem cells (Hayashi et al, 2016; pmid:26958835). To study the intracellular accumulation of S. aureus in the epidermis of DFUs, flow cytometry was performed on single-cell suspensions from full-thickness DFU tissue from type 2 diabetes patients and control skin using the mouse version of this antibody (Pastar et al, 2021; pmid:34730110).
Antibody first published in:
Geerts et al. Binding of integrin alpha6beta4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding J Cell Biol. 1999 Oct 18;147(2):417-34. PMID:10525545
Note on publication:
The recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin was investigated and it has been shown that the cytoplasmic domain of the beta4 subunit associates with an NH(2)-terminal fragment of plectin that contains the actin-binding domain (ABD).
Immunofluorescence staining of A431 cells with anti-CD104 antibody 58XB4 (Ab02885). Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of 58XB4 (Ab02885-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02885-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.