Anti-CD115/M-CSFR [AFS98]

Name: Anti-CD115/M-CSFR [AFS98]
SKU: Ab01491
Availability: InStock

Description:

Recombinant monoclonal antibody to CD115/M-CSFR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AFS98.

Clone Name:

AFS98

Published Application(s):

Blocking, WB, FC, IHC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab01491-7.1 Anti-CD115/M-CSFR [AFS98]
Mouse
Rat IgG2a
Purified
In Stock

Ab01491-2.0 Anti-CD115/M-CSFR [AFS98]
Mouse
Mouse IgG2a
Purified, VivopureX
In Stock

Ab01491-2.3 Anti-CD115/M-CSFR [AFS98]
Mouse
Mouse IgG2a
Fc Silent™
Purified
In Stock

Ab01491-23.0 Anti-CD115/M-CSFR [AFS98]
Mouse
Rabbit IgG
Purified
In Stock

Ab01491-1.6 Anti-CD115/M-CSFR [AFS98]
Mouse
Mouse Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P09581

Alternative Name(s) of Target: CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms
Immunogen: This antibody was raised against CD115.

Specificity: This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation.

Application Notes: AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).

Antibody first published in: Sudo et al. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 1995 Dec 21;11(12):2469-76. PMID:8545103
Note on publication: This article describes the generation and characterisation of the AFS98 antibody.

IF staining of RAW264.7 cells using Ab01491-23.0. Click on image for more details.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD115/M-CSFR antibody AFS98 (Ab01491) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of AFS98 (Ab01491-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01491-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of mouse splenocytes using Ab01491-23.0. Click on image for more details.

Flow cytometry using the Anti-CD115/M-CSFR antibody AFS98 (Ab01491). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of AFS98 (Ab01491-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.