United Kingdom (UK)
Recombinant monoclonal antibody to CD11b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BEAR1.
UniProt Accession Number of Target Protein: P11215
Alternative Name(s) of Target: Complement Receptor 3; CR3; BEAR-1; Integrin alpha-M; ITGAM; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor
Immunogen: The mouse version of this antibody was raised by immunizing a BALB/c mouse with purified human monocytes.
Specificity: This antibody is specific for human CB11b. CB11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. CD11b is also identical with CR-3, the receptor for the iC3b fragment of the third complement component.
Application Notes: To investigate the role of adhesion molecules in the release of histamine, a histamine release assay was performed on human basophils under influence of the mouse version of this antibody (Watanabe et al, 1992; pmid:1384863). To study the expression of complement proteins on the surface of T lymphocytes, flow cytometry was performed on human T cells using the mouse version of this antibody (Wagner et al, 2001; pmid:11298342). To determine whether PMN ADCC also would be increased by the addition of an antibody/GM-CSF fusion protein and whether this would be associated with the up-regulation and activation of Mac-1, flow cytometry was performed on LA-N-1 neuroblastoma cells using the mouse version of this antibody (Metelitsa et al, 2002; pmid:12010822). Flow cytometry was performed on human full blood to create a standardized test for CD11b expression in PMN cells (Latger-Cannard et al, 2004; pmid:15163315). To study the uptake of L. pneumophila in monocytes using complement proteins, flow cytometry was used on MM6 cells using the mouse version of this antibody (Weissgerber et al, 2003; pmid:12620617). Immunofluorescence on isolated PMN was performed using the mouse version of this antibody to understand the action of surface bound elastase on PMN (Zimmermann et al, 2005; pmid:15718918). To study the interaction between CR3 and phagocytosis, an interaction experiment was performed between CD16 and PBMCs using the mouse version of this antibody as a blocking antibody. Furthermore, this antibody was used to immunophenotype PBMCs (Preynat-Seauve et al, 2004; pmid:14971040).
Antibody first published in:
Watanabe et al. Effects of anti-CD11a, anti-CD11b and anti-CD18 on histamine release from human basophils primed with IL-3 Int Arch Allergy Immunol. 1992;98(4):308-10. PMID:1384863
Note on publication:
to investigate the possible role of adhesion molecules in the histamine release from human basophils primed with recombinant IL-3 (rIL-3). Anti-IgE monoclonal antibody (0.1 microgram/ml) did not induce apparent histamine release by itself, however, remarkable histamine release was induced from rIL-3-primed human basophils triggered by anti-IgE.