- Ab00162-7.1 Anti-CD169 [3D6.112 (recombinant version)]
- Rat IgG2a
- In Stock
- Ab00162-23.0 Anti-CD169 [3D6.112 (recombinant version)]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to CD169. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3D6.112 (recombinant version).
UniProt Accession Number of Target Protein: Q62230
Alternative Name(s) of Target: Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1
Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
Specificity: This antibody specifically recognize mouse CD169, which is a 180-185-kDa member of the Siglec family of macrophage-specific cell adhesion and endocytic receptors interacting with sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery.
Antibody first published in:
Crocker PR, Kelm S, Dubois C, Martin B, McWilliam AS, Shotton DM, Paulson JC, Gordon S. Purification and properties of sialoadhesin, a sialic acid-binding receptor of murine tissue macrophages. EMBO J. 1991 Jul;10(7):1661-9. PMID:2050106
Note on publication: Used for the purfication and ligand specifity studies of sialoadhesin isolated from mouse spleen.
Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody Ab00162-7.1 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.