United Kingdom (UK)
Recombinant monoclonal antibody to CD19. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MB19-1.
UniProt Accession Number of Target Protein: P25918
Alternative Name(s) of Target: B-lymphocyte antigen CD19; Differentiation antigen CD19; B-lymphocyte surface antigen B4; B4; CD19 molecule; CD19 antigen
Immunogen: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.
Specificity: This antibody recognizes murine MB19-1 (CD19), a transmembrane protein present in B lymphocytes that regulates its activation. It is required for proper differentiation of B-1 cells.
Application Notes: This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996; PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018; PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017; PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009; PMID: 19907079).
Antibody first published in:
S Sato, N Ono, D A Steeber, D S Pisetsky, T F Tedder CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996 Nov 15;157(10):4371-8. PMID:8906812
Note on publication:
Describes the generation of the antibody and its use in the characterization of CD19.
Immunofluorescence staining of mouse splenocytes with anti-CD19 (Ab02364) MB19-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MB19-1 (Ab02364-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02364-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD19 antibody MB19-1 (Ab02364). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of MB19-1 (Ab02364-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of mouse splenocytes with anti-CD19 (Ab02364) MB19-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MB19-1 (Ab02364-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02364-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.