- Ab00606-1.1 Anti-CD22 [RFB-4]
- Mouse IgG1
- In Stock
- Ab00606-23.0 Anti-CD22 [RFB-4]
- Rabbit IgG
- Ships in 4-5 weeks
Recombinant monoclonal antibody to CD22. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RFB-4.
UniProt Accession Number of Target Protein: P20273
Alternative Name(s) of Target: SIGLEC-2; SIGLEC2
Immunogen: Tonsil lymphocytes
Specificity: This antibody binds human CD22.
Application Notes: This antibody may be used in establishing the origin of B cell malignancies, especially discriminating between immature (bone marrow type) and peripheral B cell leukaemias and lymphomas. Suitable for use as an immunotoxin against human B cell leukaemias and lymphomas.
Antibody first published in: PMID:
Immunofluorescence staining of fixed Daudi cells with anti-CD22 antibody RFB-4 (Ab00606) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of RFB-4 (Ab00606-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00606-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.