Anti-CD24 [YBM34.3]

Description:

Recombinant monoclonal antibody to CD24. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from Waldman YBM34.3.

Clone Name:

YBM34.3

Published Application(s):

Published Species Reactivity:

Mouse

UniProt Accession Number of Target Protein: P24807

Alternative Name(s) of Target: Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen; YBM 34.3.6; YBM 34.3.6.1.3.01
Immunogen: The original antibody was generated by immunzing DA rats with CBA/Ca mouse bone marrow cells.

Specificity: This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.

Application Notes: This antibody can be used for the study of CD24 expression on cell surfaces using flow cytometry (PMID: 6608694). This antibody binds CD24 expressed on B lymphocytes, neutrophils, red cells, monocytes and cells of the erythroid lineage. This antibody was used in a study to separate hemopoietic progenitor cells from adult mouse marrow and segregate erythroid progenitor cells at distinct stages of differentiation on the basis of their cell surface antigens (PMID: 6337654).

Antibody first published in: Hale et al. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. 1985 May;134(5):3056-61. PMID:3980990
Note on publication: This paper evaluates the ability of rat antibodies to promote ADCC activity with human effector cells.