- Ab00561-7.1 Anti-CD244 [OX-122]
- Rat IgG2a
- In Stock
- Ab00561-2.0 Anti-CD244 [OX-122]
- Mouse IgG2a
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- Ab00561-23.0 Anti-CD244 [OX-122]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to CD244. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-122.
UniProt Accession Number of Target Protein: Q07763
Alternative Name(s) of Target: 2B4; Natural killer cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; Non-MHC restricted killing associated; SLAM family member 4; SLAMF4; Signaling lymphocytic activation molecule 4; OX 122
Specificity: This antibody binds to mouse CD244, which is expressed on natural killer (NK) cells, NKT cells, dendritic cells and CD8+ T cells.
Antibody first published in: PMID:
Immunofluorescence staining of fixed mouse splenocytes with anti-CD244 antibody OX-122 (Ab00561) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of OX-122 (Ab00561-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00561-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-23.0) followed by Alexa Fluor® 488 secondary antibody.
Western Blot using anti-CD244 antibody OX-122 (Ab00561) Mouse thymus lysate (A) and mouse spleen lysate (B) samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of OX-122 (Ab00561-23.0) at 2 and 1 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD244 is 44.8kDa.