United Kingdom (UK)
Recombinant monoclonal antibody to CD28. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1C6.
UniProt Accession Number of Target Protein: Q9N0N8
Alternative Name(s) of Target: TP44; Costimulatory molecule B7 receptor CD28; T-cell costimulatory molecule CD28
Immunogen: Canine CD28.
Application Notes: The antibody binds specifically to canine CD28, a self self surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6. The 1C6 monoclonal antibody has antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte mediated killing. The use of the monoclonal antibody 1C6 for the neutralisation of CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT).
Antibody first published in:
Scott S. Graves, Diane M. Stone, Carol Loretz, Laura J. Peterson, Marina Lesnikova, Billanna Hwang, George E. Georges, Richard Nash and Rainer Storb. Antagonistic and agonistic anti-canine CD28 monoclonal antibodies: tools for allogeneic transplantation. Transplantation. 2011 Apr 27; 91(8): 833–840. PMID:21343872
Note on publication:
Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
Flow-cytometry using the anti-CD28 antibody 1C6 (Ab00472) Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (Ab00472-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.