United Kingdom (UK)
Recombinant monoclonal antibody to CD35. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HB-8592.
UniProt Accession Number of Target Protein: P17927
Alternative Name(s) of Target: CR1; C3BR; C4BR; HB8592; HB 8592
Immunogen: Animals were immunized with purified CR1 from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Specificity: The antibody binds to a site distinct from the C3b binding site, and can be used to measure the number of receptors despite occupancy by ligand.
Application Notes: This antibody has been used for analysis of the expression of the CR1 receptor on human leukemia-derived CD4+ T cell lines by flow cytometry (Yaskanin 1995).
Antibody first published in:
P. H. HART, C. A. JONES & J. J, FINLAY-JONES Peritoneal macrophages during peritonitis. Phenotypic studies Clin. exp. Immunol (1992) 88, 484-491 PMID:1606734
Note on publication:
Macrophage phenolypicprofile was compared with that of normal peripheral blood monocytes, anticipating that the phenotypic profile reflects the functional activity of the cell.
Immunofluorescence staining of Jurkat cells using anti-CD35 antibody (Ab00897) HB-8592. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of HB-8592 (Ab00897-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00897-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-CD35 antibody HB-8592 (Ab00897). Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (Ab00897-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.