- Ab00113-1.1 Anti-CD4 [W3/25]
- Mouse IgG1
- In Stock
- Ab00113-23.0 Anti-CD4 [W3/25]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to CD4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma W3/25.
UniProt Accession Number of Target Protein: P05540
Alternative Name(s) of Target: T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD4
Immunogen: Membraneous glycoproteins from rat thymocytes.
Specificity: Recognises a 50kDa Cell surface glycoprotein expressed by helper T cells - found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages.
Application Notes: This antibody binds to rat CD4 and is able to suppress IL2 production in the Mixed Lymphocyte Reaction Assay.
Antibody first published in:
Williams AF, Galfrè G, Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663-73. PMID:303545
Note on publication: Describes the generation of the antibody, shows it recognised rat thymocytes, and analysed by immunoblotting and fluorescence activated cell sorter.
Immunohistochemical staining of rat spleen using anti-CD4 antibody Ab001113 Formalin fixed rat spleen slices were were stained with Ab00113-23.0 at 3 µg/ml.