United Kingdom (UK)
Recombinant monoclonal antibody to CD47. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mIAP301.
UniProt Accession Number of Target Protein: Q61735
Alternative Name(s) of Target: IAP; Leukocyte surface antigen CD47; Integrin-associated protein; integrin associated protein; MIAP 301; MIAP-301
Immunogen: MIAP301 was prepared by immunizing rats with intact CD47 purified from mouse placenta.
Specificity: MIAP301 binds specifically to the extracellular domain of CD47 (exon 2 that encodes both the predicted signal peptide clevage site and the entire Ig domain). CD47 plays a role in cell adhesion by acting as an adhesion receptor for THBS1 on platelets and in (beta-3) integrin modulation. Functions as a SIRPA receptor - binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 also interacts with SIRPG which mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Plays an important role in memory formation and synaptic plasticity in the hippocampus.
Application Notes: MIAP301 can block murine CD47 interactions with SIRPα and functionally blocks CD47 in vivo in murine soft tissue ischemia and skin graft models (Soto-Pantoja et al. 2012). This antibody has also been used for FC (Olsson et al. Blood. 2005 May 1; 105(9): 3577–3582.). MIAP301 has also been shown to block KCl-evoked glutamate release from the hippocampus in mice, inhibiting glutamate release and thus impair memory retention (Chang et al. 2001).
Antibody first published in:
Lindberg et al. Decreased Resistance to Bacterial Infection and Granulocyte Defects in lAP-Deficient Mice Science. 1996 Nov 1;274(5288):795-8. PMID:8864123
Note on publication:
Describes the use of MIAP301 in determining CD47 tissue distribution and to ensure no functional fragment of CD47 was expressed in CD47 -/- KO mice.
Immunofluorescence staining of fixed L929 cells with anti-CD47 antibody mIAP301 (Ab01569) Immunofluorescence analysis of paraformaldehyde fixed L929 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of mIAP301 (Ab01569-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01569-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD47 antibody mIAP301 (Ab01569). Paraformaldehyde fixed L929 cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of mIAP301 (Ab01569-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.