Anti-CD52 [Campath-1H]

Description:

Recombinant monoclonal antibody to CD52. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody Campath-1H.

Clone Name:

Campath-1H

Published Application(s):

Cytotoxicity Assay, IHC-P, in vivo, WB, ELISA, FC, IHC-Fr

Published Species Reactivity:

Human, Rhesus Monkey, Cynomolgus Monkey

9

Products based on this clone:

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Ab00124-10.0 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Human IgG1
Purified
In Stock

Ab00124-8.1 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Rat IgG2b
Purified
In Stock

Ab00124-8.4 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Rat IgG2b
Fc Silent™
Purified
In Stock

Ab00124-10.7 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Human F(ab)2
AbFab2™ His-Tagged
Purified
In Stock

Ab00124-23.0 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Rabbit IgG
Purified
In Stock

Ab00124-10.3 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Human IgG1
Fc Silent™
Purified
In Stock

Ab00124-35.0 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Cynomolgus monkey IgG1
Purified
Ships in 4-5 weeks

Ab00124-26.0 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Rhesus macaque IgG1
Purified
Ships in 4-5 weeks

Ab00124-10.2 Anti-CD52 [Campath-1H]
Human, Rhesus Monkey, Cynomolgus Monkey
Human IgG1
Aglycosylated
Purified
In Stock

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UniProt Accession Number of Target Protein: P31358

Alternative Name(s) of Target: CDW52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; YTH66.9HL; Alemtuzumab
Immunogen: Human lymphocytes.

Specificity: Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Antibody first published in: Riechmann L, Clark M, Waldmann H, Winter G. Reshaping human antibodies for therapy. Nature. 1988 Mar 24;332(6162):323-7. PMID:3127726
Note on publication: Describes the reshaping of the rat IgG2b antibody to human IgG1 and shows that it recognises human CD52 antigen.

Binding Curve

Immunofluorescence imaging on fixed rat spleen tissue using Ab00124. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (Ab00124) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (Ab00124-10.0) at 5 µg/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; Ab00124-10.0 (FITC), DAPI and merged channels.

Binding Curve

IHC on rat epididymis tissue using Ab00124-10.0. Click on image for more details.

Binding Curve

Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Binding Curve

IHC on rat spleen tissue using Ab00124-10.0. Click on image for more details.

Binding Curve

Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Binding Curve

IHC on rat thymus tissue using Ab00124-10.0. Click on image for more details.

Binding Curve

Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Binding Curve

Flow-cytometry on rhesus monkey lymphocytes using Ab00124. Click on image for more details.

Binding Curve

Flow-cytometry using anti-CD52 antibody Campath-1H (Ab00125) Rhesus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Campath-1H (Ab00125-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Binding Curve

Flow-cytometry on cynomolgus monkey lymphocytes using Ab00124. Click on image for more details.

Binding Curve

Flow-cytometry using anti-CD52 antibody Campath-1H (Ab00124) Cynomolgus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Campath-1H (Ab00728-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Binding Curve

IF staining of rat thymus using Ab00124. Click on image for more details.

Binding Curve

Immunofluorescent staining of rat thymus using anti-CD52 antibody Ab00124 Formaldehyde-fixed rat thymus slices were stained with Ab00124-10.0 at 5 µg/ml and detected with a FITC-conjugated secondary antibody. Ab00124 shows cross-reactivity with rat.

Binding Curve

IHC on human thyroid cancer using Ab00124. Click on image for more details.

Binding Curve

Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody Ab00124 Formalin fixed human thyroid cancer slices were were stained with a Ab00124-10.0 at 5 µg/ml.

Binding Curve

Flow-cytometry on rhesus monkey lymphocytes using Ab00124. Click on image for more details.

Binding Curve

Flow-cytometry using anti-CD52 antibody Campath-1H (Ab00125) Rhesus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Campath-1H (Ab00125-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Binding Curve

Immunofluorescence imaging on fixed rat spleen tissue using Ab00124. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (Ab00124) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (Ab00124-10.0) at 5 µg/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; Ab00124-10.0 (FITC), DAPI and merged channels.