United Kingdom (UK)
Recombinant monoclonal antibody to CD56. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N901.
UniProt Accession Number of Target Protein: P13591
Alternative Name(s) of Target: NCAM1; NCAM; NCAM-1; Neural cell adhesion molecule 1
Immunogen: The original antibody was generated by immunizing mice with human chronic myelogenous leukemia (CML) cells.
Specificity: This antibody binds CD56, present on large granular lymphocytes including natural killer cells. This antibody does not react with monocytes, granulocytes, B cells, erythrocytes, or platelets.
Application Notes: This antibody does not block the natural killer cell activity and can be used to rapidly purify functional NK cells (PMID:6574190). This antibody was able to precipitate a band at approx. 200-250 kdalton protein in a immunoprecipitation assay. This antibody successfully blocked the binding of anti-NKH1A antibody in an indirect immunofluorescence assay (PMID:3884668). This antibody is also recommended to be used in the study of cell surface antigens of the natural cells (PMID: 3875369). Conjugate of DC1 with murine and humanizsed versions of N901 was found to be potent and effective in selective killing of target cells in culture (PMID: 7545085). THis antibody can also be used for the separation of natural killer cells in a flow cytometry (PMID: 15985539).
Antibody first published in:
Griffin et al. Characterization of an antigen expressed by human natural killer cells. J Immunol (1983);130(6):2947-51. PMID:6574190
Note on publication:
Describes the characterization of the antigen present on human natural killer cells using this antibody.
Immunofluorescence staining of Kelly cells with Anti-CD56 (Ab02209) N901 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of N901 (Ab02209-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02209-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.