United Kingdom (UK)
Recombinant monoclonal antibody to CD98. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma EP3-1.
UniProt Accession Number of Target Protein: Q01650
Alternative Name(s) of Target: LAT1; E16; MPE16; SLC7A5
Immunogen: Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
Specificity: Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2, but showed no reactivity to live HeLa cells by IF.
Application Notes: CD98 may be a target for T or B cell immune responses against tumour cells in cancer patients (Itoh 2008).
Antibody first published in:
Itoh Antibody epitope peptides as potential inducers of IgG antibodies against CD98 oncoprotein. Cancer Sci. 2009 Jan;100(1):126-31. PMID:19018757
Note on publication:
Describes generation of antibody and potential for therapeutic use in initiating immune response against cancer.
Immunofluorescence staining of MCF7 cells with antiCD98 (Ab00898) EP3-1 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (Ab00898-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00898-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD98 antibody EP3-1 (Ab00898). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of EP3-1 (Ab00898-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of MCF7 cells with antiCD98 (Ab00898) EP3-1 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (Ab00898-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00898-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.