- Ab00641-2.0 Anti-CMV glycoprotein gB [HCMV37]
- Mouse IgG2a
- In Stock
- Ab00641-23.0 Anti-CMV glycoprotein gB [HCMV37]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to CMV glycoprotein gB. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HCMV37.
Alternative Name(s) of Target: Human cytomegalovirus glycoproteins gB
Immunogen: The original antibody was generated by immunization of rabbit with recombinant HCMV gB-VAC virus.
Specificity: This antibody binds envelope glycoprotein B of the human cytomegalovirus, that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells.
Application Notes: This antibody immunoprecipitates gB from HCMV infected cells and neutralizes HCMV infectivity in vitro. Immunofluorescence was used to study whether HCMV gB synthesized in infected cells is transported to the cell surface (PMID: 3024973). Humanized version of this antibody was made by grafting technique. Significant restoration of binding was obtained by substitution of human VH amino acids Thr28, Phe29, Ser30 with murine residues Ser28, Ile29, Thr30, in conjunction with the position 94 change (PMID: 7772562). This antibody was used in detection of gB in an ELISA (PMID: 22836657).
Antibody first published in:
Tempest et al. Identification of framework residues required to restore antigen binding during reshaping of a monoclonal antibody against the glycoprotein gB of human cytomegalovirus. Int J Biol Macromol. 1995 Feb;17(1):37-42. PMID:7772562
Note on publication: Describes characterisation of the structural requirements of HCMV37 essential for antigen binding.