United Kingdom (UK)
Recombinant monoclonal antibody to Coat protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody scFvB9.
UniProt Accession Number of Target Protein: A0A0H4SQY8
Alternative Name(s) of Target: CpCDV; CpCDV coat protein; CpCDV capsid protein; V1; CP; capsid protein; CpCDV-scFvB9
Immunogen: The original antibody was isolated from Tomlinson phage display antibody library by screening against coat protein of Chickpea chlorotic dwarf virus (CpCDV) expressed in Escherichia coli strain Rosetta (DE3).
Specificity: This antibody binds the coat protein of Chickpea chlorotic dwarf virus (CpCDV). CpCDV is a monopartite, single-stranded circular DNA virus in the genus Mastrevirus and family Geminiviridae. It is transmitted by Orosius albicinctus in a circulative (persistent) and nonpropagative manner. It is the causative agent for Chickpea stunt disease (CSD) that is a threat to chickpea production leading to yield losses of 75⁻95%. Symptoms of CSD include very small leaves, intense discoloration (yellowing (kabuli type) and reddening (desi type)), and bushy stunted appearance of the plant.
Application Notes: The affinity characterization of this antibody for Chickpea chlorotic dwarf virus (CpCDV) was done using ELISA. This antibody reacted with recombinant coat protein of CpCDV in a western blot (PMID: 32970278).
Antibody first published in:
Raeisi et al. Isolation of single-chain variable fragment (scFv) antibodies for detection of Chickpea chlorotic dwarf virus (CpCDV) by phage display. Arch Virol. 2020 Dec;165(12):2789-2798. PMID:32970278
Note on publication:
This paper describes the development and characterization of a recombinant monoclonal antibody for CpCDV which can be used as a diagnostic tool.