United Kingdom (UK)
Recombinant monoclonal antibody to Cry1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3H9.
UniProt Accession Number of Target Protein: Q03746; P0A366; P0A370; P05068; D0EI56; Q93T75
Alternative Name(s) of Target: Cry1 toxins; Cry1Aa; Cry1Ab; Cry1Ac; Cry1B; Cry1C; Cry1F; Pesticidal crystal protein Cry1Aa; 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIA(a); Pesticidal crystal protein Cry1Fa; 134 kDa crystal protein; Insecticidal delta-endotoxin CryIF(a); Pesticidal crystal protein Cry1Ab; 130 kDa crystal protein; Insecticidal delta-endotoxin CryIA(b); Pesticidal crystal protein Cry1Ac;133 kDa crystal protein; Insecticidal delta-endotoxin CryIA(c)
Immunogen: The original antibody was generated by immunizing Balb/c mice with a mixture of six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F) followed by antibody library construction and phage display-based selection.
Specificity: The antibody binds to Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F). The antibody does not cross react with Cry2A and vip3.
Application Notes: The specificity of the scFv antibody for the six Cry1 toxins was confirmed by ELISA. The scFv fragment was used to establish the double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) for detecting six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F). The lowest detectable limits (LOD) and the lowest quantitative limits (LOQ) were 3.14–11.07 and 8.22–39.44 ng mL−1 , respectively, with the correlation coefficient higher than 0.997. The average recoveries of Cry1 toxins from spiked rice leaf samples were ranged from 84 to 95%, with coefficient of variation (CV) less than 8.2%, showing good accuracy for the multi-residue determination of six Cry1 toxins in agricultural samples (Dong et al., 2018; PMID: 29484477).
Antibody first published in:
Dong et al. Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library. Appl Microbiol Biotechnol. 2018 Apr;102(7):3363-3374. PMID:29484477
Note on publication:
The paper describes the generation and characterization of the antibody.