United Kingdom (UK)
Recombinant monoclonal antibody to cytochrome C. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R1D3.
UniProt Accession Number of Target Protein: P62897
Alternative Name(s) of Target: cyt c; Cytochrome c somatic
Specificity: The antibody is specific for cytochrome c. The antibody binds to a variety of oxidatively modified cyt c species, such as nitrated at Tyr74 or oxidized at Met80, which lose the sixth heme ligation (Fe-Met80); it does not bind to several cyt c phospho- and acetyl-mimetics.
Application Notes: The antibody was employed to identify alternative conformations of cyt c in cells under oxidant- or senescence-induced challenge as confirmed by immunocytochemistry (Tomasina et al., 2022; PMID: 36378644).
Antibody first published in:
Tomasina et al. De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells Proc Natl Acad Sci U S A. 2022 Nov 22;119(47):e2213432119. PMID:36378644
Note on publication:
The paper describes the sequencing of the original antibody