- Ab03190-23.0 Anti-Cytokeratin 20 [10A2]
- Rabbit IgG
- In Stock
- Ab03190-2.0 Anti-Cytokeratin 20 [10A2]
- Mouse IgG2a
- In Stock
- Ab03190-10.0 Anti-Cytokeratin 20 [10A2]
- Human IgG1
- Ships in 4-5 weeks
Recombinant monoclonal antibody to Cytokeratin 20. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10A2.
UniProt Accession Number of Target Protein: P35900
Alternative Name(s) of Target: CK20; CK-20; K20; KRT20; Cytokeratin-20; Keratin-20; Keratin type I cytoskeletal 20 ;Protein IT; CGMCC NO 20764; KS20.8
Immunogen: The original antibody was generated by immunizing female ICR mice with recombinant human CK20 histidine-tagged fusion protein in Freund's complete adjuvant.
Specificity: This antibody binds human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine.
Application Notes: The binding characterization of this antibody was done using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92 × 10^9 M^(-1). This antibody was capable of detecting recombinant CK20 protein in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of stomach epithelial tissues and gastric adenocarcinomas (CN113583120).
Antibody first published in: PMID: