- Ab00158-1.1 Anti-desmoglein-3 [PVB28]
- Mouse IgG1
- In Stock
- Ab00158-13.0 Anti-desmoglein-3 [PVB28]
- Human IgG4
- In Stock
Recombinant monoclonal antibody to desmoglein-3. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the EBV transformed human B cell clone PVB28.
UniProt Accession Number of Target Protein: P32926
Alternative Name(s) of Target: Pemphigus Vulgaris Ag; 130 kDa pemphigus vulgaris antigen; PVA; Cadherin family member 6; DSG3; CDHF6
Immunogen: Made by immortalizing IgG-expressing B cells from Pemphigus vulgaris (PV) patients.
Specificity: This antibody specifically recognizes a conformational epitope in the EC2 domain of human desmoglein-3 (DSG This antibody was isolated as a human IgG4 from a patient with mucocutaneous pemphigus vulgaris and uses the VH4-39*07, D6-13*01, JH4*02, VL3-15*01and JL2*01. This antibody has in vitro and in vivo pathogenic activity, as assessed in a keratinocyte dissociation assay and by the formation of microscopic blisters in newborn mice. This antibody crossreacts with murine DSG-3.
Antibody first published in:
Di Zenzo,G. et al. Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface. J Clin Invest 122, 3781–3790 (2012). PMID:22996451
Note on publication: Describes the isolation and analysis of a panel of human antibodies from patients - inc data on ELISA, IF and in vitro and in vivo pathogenic activity assays.
(A) Cryosections of human skin stained with biotinylated DSG3-specific monoclonal antibodies (Ab00153, Ab00155, Ab00158 and Ab00156). (B) Staining of human epidermis with biotinylated PVB28 (Ab00158) in the presence (with) or absence (w/o) of Ca2+. (C) Binding of a panel of pemphigus auto-antibodies to DSG3-coated ELISA plates in the presence or absence of EDTA.