United Kingdom (UK)
Recombinant monoclonal antibody to E2/E3E2 envelope protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody WD11F.
Alternative Name(s) of Target: Western equine encephalitis virus
Immunogen: An immune phage display library derived from the peripheral blood lymphocytes of a llama that had been immunized with an equine vaccine that includes killed WEEV (West Nile Innovator + VEWT) was generated. The antibody was selected by panning the library against recombinant envelope (E2/E3E2) protein from WEEV.
Specificity: The antibody is specific for E2/E3E2 envelope protein.Two envelope proteins, glycoproteins E1 and E2, associate as trimers of E1-E2 dimers on the viral surface, and make up 80 spikes on the viral surface.
Application Notes: The binding affinity of the VHH fragment for the WEEV E3E2 recombinant protein was measured by surface plasmon resonance (Kd= 7.2 ×10^−9 M). The ability of the antibody to detect the recombinant protein in a MagPlex sandwich immunoassay was confirmed (Liu et al., 2018; PMID: 31544894).
Antibody first published in:
Liu et al. Selection of Single-Domain Antibodies towards Western Equine Encephalitis Virus Antibodies (Basel). 2018 Dec; 7(4): 44. PMID:31544894
Note on publication:
The original paper describes the selection and characterization of single-domain antibodies towards the E2/E3E2 envelope protein of the Western equine encephalitis virus (WEEV).