- Ab00303-23.0 Anti-ERBB2 [SAIC-02A-7]
- Rabbit IgG
- In Stock
- Ab00303-1.1 Anti-ERBB2 [SAIC-02A-7]
- Mouse IgG1
- In Stock
Recombinant monoclonal antibody to ERBB2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-02A-7.
UniProt Accession Number of Target Protein: P04626
Alternative Name(s) of Target: HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2
Immunogen: Peptide "GLQSLPTHDPSPLQR" derived from the receptor tyrosine kinase erbB2 conjugated to KLH.
Specificity: Recognises human ERBB2.
Application Notes: Original data characterizing this antibody may be found here .
Antibody first published in:
Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.
Immunofluorescence staining of MCF7 cells using anti-ERBB2 antibody (Ab00303) SAIC-02A-7. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of SAIC-02A-7 (Ab00303-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00303-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-ERBB2 antibody SAIC-02A-7 (Ab00303). MCF7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of SAIC-02A-7 (Ab00303-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.