Anti-ESAT-6 [EA-S3]

Name: Anti-ESAT-6 [EA-S3]
SKU: Ab01249
Availability: InStock

Description:

Recombinant monoclonal antibody to ESAT-6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the ribosome display antibody EA-S3.

Clone Name:

EA-S3

Published Application(s):

ELISA

Published Species Reactivity:

Mycobacterium tuberculosis

Products based on this clone:

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Ab01249-1.1 Anti-ESAT-6 [EA-S3]
Mycobacterium tuberculosis
Mouse IgG1
Purified
Ships in 4-5 weeks

Ab01249-23.0 Anti-ESAT-6 [EA-S3]
Mycobacterium tuberculosis
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: B5TV89

Immunogen: BALB/c mice were immunised with ESAT-6 protein and RNA extracted from the spleen to produce a scFv library. Eukaryotic ribosome display was used to select EA-S3.

Specificity: ESAT-6 is a potent T-cell antigen and suggested as a diagnostic biomarker for TB infection.

Application Notes: ELISA was used to confirm the specificity of EA-S3 for ESAT-6; ELISA also determined the affinity constant of EA-S3 for ESAT-6 as 3.74x10^8 M^-1 (Ahangarzadeh et al, 2017).

Antibody first published in: Ahangarzadeh et al, 2018. Selection of single-chain variable fragments specific for Mycobacterium tuberculosis ESAT-6 antigen using ribosome display. Iran J Basic Med Sci. 2017 Mar;20(3):327-333 PMID:28392906
Note on publication: Describes the generation of EA-S3 from a ribosome display library and its characterisation through ELISA.