Anti-Factor VIII [EL-14]

Description:

Recombinant monoclonal antibody to Factor VIII. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody EL-14.

Clone Name:

EL-14

Published Application(s):

functional assay, inhibit, IP

Published Species Reactivity:

Human

Products based on this clone:

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Ab04011-23.0 Anti-Factor VIII [EL-14]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

Ab04011-1.1 Anti-Factor VIII [EL-14]
Human
Mouse IgG1
Purified
Ships in 5-6 weeks

Ab04011-10.0 Anti-Factor VIII [EL-14]
Human
Human IgG1
Purified
Ships in 5-6 weeks

Ab04011-10.3 Anti-Factor VIII [EL-14]
Human
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

Ab04011-30.11 Anti-Factor VIII [EL-14]
Human
scFv fragment (His)
ScFv
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P00451

Alternative Name(s) of Target: AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Immunogen: The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.

Specificity: This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.

Application Notes: The binding characterization of this antibody towards radiolabeled FVIII light chain and the C2 domain of factor VIII was done using immunoprecipitation. The inhibitory activity of this antibody against factor VIII was determined using a one-stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462). The scFv version of this antibody bound factor VIII light chain with a dissociation constant of Kd = 1.1 nM (PMID: 11929772).

Antibody first published in: van den Brink et al. Human antibodies with specificity for the C2 domain of factor VIII are derived from VH1 germline genes Blood. 2000 Jan 15;95(2):558-63. PMID:10627462
Note on publication: Describes the generation and epitope mapping of antibodies against C2 domain of factor VIII.