United Kingdom (UK)
Recombinant monoclonal antibody to Fc epsilon R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma IE7.
UniProt Accession Number of Target Protein: P30273
Alternative Name(s) of Target: FceRI; IgE receptor
Immunogen: Recombinant human protein, N-terminus.
Specificity: This antibody binds to human Fc epsilon receptor, but does not compete with IgE binding.
Application Notes: IE7 binds to human FceRIa, but does not compete with IgE binding or binding of the antibody IB10 (Ab01396).
Antibody first published in: PMID:
Immunofluorescence staining of U937 cells using anti-Fc epsilon R antibody (Ab01397) IE7. Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of IE7 (Ab01397-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01397-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the Anti-Fc epsilon R antibody IE7 (Ab01397). U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of IE7 (Ab01397-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.