United Kingdom (UK)
Recombinant monoclonal antibody to Feline Calicivirus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1D7.
UniProt Accession Number of Target Protein: P27405
Alternative Name(s) of Target: FCV; Capsid protein; Coat protein; CP; VP1
Immunogen: The original antibody was raised by immunizing BALB/c mice with feline calcivirus infected CRFK cells. After two weeks, mouse lymphocytes and mouse myeloma cells (P3U1) were cell-fused with polyethylene glycol to prepare hybridomas.
Specificity: 1D7 is capable of neutralizing all these FCV strains: F4, F1, F3, F5, F7, F6, F8, F11, F14, F25, FC, FR, FPL, CFI, FJ, 227, 337, and KDC. Does not recognise the denatured antigen.
Application Notes: This antibody binds and neutralizes the feline calicivirus. It is capable of detecting the virus in an ELISA. Neutralisation assays confirmed 1D7 neutralises feline calicivirus at 208.2 ng/ml. The Ki of 1D7 is 1 μg/ml. This antibody is not capable of recognizing the denatured FCV antigen in an immunoblot assay. It was considered that the 1D7 epitope might exist in a conserved region on the capsid protein because of the ability of 1D7 to neutralize all the strains of FCV tested (PMID:1708228).
Antibody first published in:
Tohya et al. Neutralizing epitopes of feline calicivirus. Arch Virol (1991); 117(3-4):173-81. PMID:1708228
Note on publication:
Describes the generation of this antibody along with others and using them to analyze neutralizing epitopes of the F4 strain of FCV.