Anti-GA24/19 [A24]

Name: Anti-GA24/19 [A24]
SKU: Ab01183
Availability: InStock

Description:

Recombinant monoclonal antibody to GA24/19. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A24.

Clone Name:

A24

Published Application(s):

RIA, transgenic studies

Published Species Reactivity:

Tobacco

Products based on this clone:

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Ab01183-1.1 Anti-GA24/19 [A24]
Tobacco
Mouse IgG1
Purified
Ships in 5-6 weeks

Ab01183-23.0 Anti-GA24/19 [A24]
Tobacco
Rabbit IgG
Purified
Ships in 5-6 weeks

Immunogen: An scFv was prepared by generating a hybridoma cell line which produced an anti GA24/19 monoclonal antibody, from which the antibody genes were cloned to construct an anti-GA14/19 scFv gene.

Specificity: The anti-GA24/19 monoclonal antibody showed specific reactivity to GA24, GA19 and GA15. These are precursors to bioactive gibberellins.

Application Notes: RIA demonstrated the specific binding of A24 to GA24. The sequence was cloned into expression cassettes for expression in tobacco plants; these plants showed reduced growth (shorter stature) (Shimada et al, 1999).

Antibody first published in: Shimada et al, 1999. Expression of a functional single-chain antibody against GA24/19 in transgenic tobacco. Biosci Biotechnol Biochem. 1999 Apr;63(4):779-83. PMID:
Note on publication: Describes the original generation of A24 and its application to transgenic plant studies.