Anti-GABAR [1F4]

Description:

Recombinant monoclonal antibody to GABAR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1F4.

Clone Name:

1F4

Published Application(s):

crystallography, FSEC

Published Species Reactivity:

Human

6

Products based on this clone:

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Ab01411-3.0 Anti-GABAR [1F4]
Human
Mouse IgG2b
Purified
In Stock

Ab01411-3.3 Anti-GABAR [1F4]
Human
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01411-23.0 Anti-GABAR [1F4]
Human
Rabbit IgG
Purified
In Stock

Ab01411-10.0 Anti-GABAR [1F4]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01411-10.3 Anti-GABAR [1F4]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01411-1.6 Anti-GABAR [1F4]
Human
Mouse Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

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UniProt Accession Number of Target Protein: P14867; P47870; P18507

Alternative Name(s) of Target: Gamma-aminobutyric acid receptor A; Gamma-aminobutyric acid receptor; Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A); GABA-A receptor; α1β2γ2 GABA-A receptor
Immunogen: This antibody was raised against α1β2γ2 GABA-A receptor.

Specificity: This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50% of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy.

Application Notes: FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).

Antibody first published in: Zhu et al. Structure of a human synaptic GABA-A receptor Nature. 2018 Jul;559(7712):67-72. doi: 10.1038/s41586-018-0255-3. Epub 2018 Jun 27. PMID:29950725
Note on publication: This article describes the generation of 1F4 antibody as well as Fab fragments derived from it for the pupose of puryfying and analysing the structure of α1β2γ2 GABA-A receptor.

Binding Curve

IF staining of U251 cells using Ab01411-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed U251 cells with anti-GABAR antibody 1F4 (Ab01411) Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1F4 (Ab01411-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01411-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.