Anti-Gamma Delta TCR [G5-4]

Description:

Recombinant monoclonal antibody to Gamma Delta TCR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma G5-4.

Clone Name:

G5-4

Published Application(s):

functional assay, FC

Published Species Reactivity:

Human

Products based on this clone:

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Ab03990-10.3 Anti-Gamma Delta TCR [G5-4]
Human
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

Ab03990-23.0 Anti-Gamma Delta TCR [G5-4]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

Ab03990-2.3 Anti-Gamma Delta TCR [G5-4]
Human
Mouse IgG2a
Fc Silent™
Purified
Ships in 5-6 weeks

Ab03990-2.0 Anti-Gamma Delta TCR [G5-4]
Human
Mouse IgG2a
Purified
Ships in 5-6 weeks

Ab03990-10.0 Anti-Gamma Delta TCR [G5-4]
Human
Human IgG1
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P04234; P09693

Alternative Name(s) of Target: TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G
Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant γ9δ2 (OT3)-Fc fusion protein as an immunogen.

Specificity: This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.

Application Notes: The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma-delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85% when the effect-to-target ratio is 5:1 (CN103130894).

Antibody first published in: PMID: