United Kingdom (UK)
Recombinant monoclonal antibody to Glomerular antigen. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E5-1.
Alternative Name(s) of Target: GA
Immunogen: This antibody was generated from renal mononuclear, Ab-producing cells (AbPCs) derived from diseased MRL/pr mouse kidneys. MRL/pr mice spontanously develop an automimmune syndrome associated with autoantibody production, and act as models of human systemic lupus erythematosus (SLE).
Specificity: This antibody displays polyreactive binding to GA, ssDNA, dsDNA and IgG. It does not bind histones or Sm proteins.
Application Notes: The binding specificity of this antibody has been confirmed in ELISA and Western blot analysis (Sekine et al, 2004). Intravenous administration of this antibody to normal mice results in glomerular binding, as revealed by immunofluoescence analysis, suggesting that the production of glomerular-binding autoantibodies by renal mononuclear cells conributes to the developmet of lupus nephritis (Sekine et al, 2004).
Antibody first published in:
Sekine et al. Enrichment of anti-glomerular antigen antibody-producing cells in the kidneys of MRL/MpJ-Fas(lpr) mice. J Immunol. 2004 Mar 15;172(6):3913-21. PMID:15004199
Note on publication:
Describes the original generation of this antibody from mouse models of systemic lupus erythematosus, and its characterisation in ELISA and WB analysis.