United Kingdom (UK)
Recombinant monoclonal antibody to HA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from synthetic construct AV.C4.
UniProt Accession Number of Target Protein: A5A5M5
Alternative Name(s) of Target: Hemagglutinin; Avian Influenza Virus; AIV; H5N1; H5N2; H5N3; Influenza A virus
Immunogen: The original antibody was isolated from human semi-synthetic ETH-2 phage antibody library by biopanning against H5 hemagglutinin.
Specificity: This antibody binds the hemaglutinin of the H5N1 avian influenza virus.
Application Notes: The specificity of this scFv antibody for binding H5 type of hemaglutinin was determined by ELISA. AV.C4 binds a conserved epitope shared by three different virus strains belonging to the H5 subtype, i.e. the homologous strain A/Vietnam/ 1194/04 (H5N1), and two viruses isolated form wild birds, A/Nammalr/Italy/80/93 (H5N2) and A/Mallard/Italy/208/00 (H5N3). This antibody does not cross react with H1N1 influenza virus. scFv clone AV.C4 exerted a significant inhibition of the H5N1 A/Vietnam/1194/2004 virus infection in a pseudotype-based neutralization assay. This antibody was capable of detecting a 70kDa protein corresponding to the uncleaved HA protein in a western blot assay (PMID: 19481117).
Antibody first published in:
Ascione et al. Human monoclonal antibodies in single chain fragment variable format with potent neutralization activity against influenza virus H5N1. Antiviral Res. (2009); 83(3):238-44. PMID:19481117
Note on publication:
Describes the generation of scFv format of this antibody from a phage display library and its effectiveness in neutralizing the H5N1 infection.
ELISA using AV.C4 (Ab02203) and Influenza A HA [A/Hong Kong/483/97 (H5N1)]. Binding curves of the rabbit IgG chimeric version of the Anti-HAHA antibody AV.C4 (Ab02203-23.0; blue line) and isotype control (Ab00102-23.0, anti-fluorescein; orange line) to an ELISA plate coated with Influenza A HA [A/Hong Kong/483/97 (H5N1)] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab02203 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.