Anti-HA [FC41]

Name: Anti-HA [FC41]
SKU: Ab00149
Availability: InStock

Description:

Recombinant monoclonal antibody to HA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the EBV transformed human B cell clone FC41.

Made with Antibody Sequences licensed from Humabs Biomed SA.

Clone Name:

FC41

Published Application(s):

ELISA

Published Species Reactivity:

Influenza A Virus (several subtypes)

Product based on this clone:

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Ab00149-10.0 Anti-HA [FC41]
Influenza A Virus (several subtypes)
Human IgG1
Purified
In Stock

Alternative Name(s) of Target: hemagglutinin; haemagglutinin
Immunogen: Made by immortalizing IgG-expressing B cells from individuals following vaccination with seasonal influenza vaccine, containing H1 and H3 influenza Virus subtypes.

Specificity: This antibody specifically recognizes the conformational epitope in the stem region of HA in which amino acid sequences are highly conserved in H1N1, H5N1, H6N1, and H9N2 subtypes of Influenza A Virus. This antibody uses the VH1-69*06, D1-26*01, JH4*02, VL3-10*01 and JL1*01 This antibody does not cross react with HA in H3N2 and H7N7 subtype of Influenza A virus. This antibody has no effect on haemagglutination-inhibition activity.

Antibody first published in: Corti, D. et al. Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine. J Clin Invest 120, 1663–1673 (2010). PMID:20389023
Note on publication: Describes the isolation and analysis of a panel of human antibodies from vaccinated individuals and its evaluation using Virus neutralization assays.

ELISA using Ab00149-10.0 Click on image for more details.

ELISA using FC41 (Ab00149) and Influenza A HA [A/Hong Kong/483/97 (H5N1)]. Binding curves of the human IgG chimeric version of the Anti-HA antibody FC41 (Ab00149-10.0; green line) and isotype control (Ab00102-10.0, anti-fluorescein; yellow line) to an ELISA plate coated with Influenza A HA [A/Hong Kong/483/97 (H5N1)] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab00149 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-human IgG antibody was used.