United Kingdom (UK)
Recombinant monoclonal antibody to HBsAg. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F124 scFv.
UniProt Accession Number of Target Protein: Q773S4
Alternative Name(s) of Target: Hepatitis B surface antigen; Major surface protein
Immunogen: This scFv was generated from the anti-HBsAg monoclonal antibody F124, which was raised by immunization of BALB/c mice with HBV particles, serotype ay, purified from the plasma of a chronic carrier. VH and VL cDNA isolated from the F124 hybridoma were joined by a two step fusion PCR using an oligonucleotide coding for the (Gly4Ser)3 linker, and this construct was introduced into E.coli.
Specificity: This scFv is specific for the preS2 region of the surface antigen of hepatitis B virus. Specifically, this scFv recognizes an epitope localised at the N-terminal part of the M protein and containing the preS2 residue 126.
Application Notes: In ELISA analysis, this antibody has been shown to inhibit the binding of both the F124 Fab and IgG to r-HBsAg in a dose-dependent manner (Passafiume, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.
Antibody first published in:
Passafiume et al. Sequence analysis of a monoclonal antibody specific for the preS2 region of hepatitis B surface antigen, and the cloning, expression and characterisation of its single-chain Fv construction FEBS Letters, 1998, Vol.441(3), pp.407-412 PMID:9891981
Note on publication:
Describes the original generation of this scFv, and preliminary attempts to characterise its specificity and functionality.