United Kingdom (UK)
Recombinant monoclonal antibody to HBsAg. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F124.
UniProt Accession Number of Target Protein: Q773S4
Alternative Name(s) of Target: HBV M protein; Hepatitis B virus surface antigen; Hepatitis B surface antigen; HBV surface antigen; HBV-sAg; Hepatitis B virus M protein; Major surface protein
Immunogen: BALB/c mice were immunized by injection of HBV particles serotype ay, purified from HBsAg-positive patient blood sera. Splenocytes from immunized mice were then fused with the Sp2/0-Ag myeloma cell line.
Specificity: F124 was shown to react with an epitope within the pre-S2 region of the HBV surface antigen M protein, shown by Western blot and RIA using both full viral particles and HBsAg particles (Budkowska et al, 1986). F124 is also reactive against a synthetic peptide corresponding to the N-terminal residues 120-150 (FPAGGSSSGTVNPVLTTASPL) of the pre-S2 region (Ni et al, 2010). Hepatitis B is one of the most common infectious diseases on a global scale, with infection associated with both acute and chronic liver inflammation and implicated in up to half of all occurring hepatocellular carcinomas.
Application Notes: F124 was used as a solid-phase coating for RIAs detecting HBV particles, resulting in particle immobilisation by binding the pre-S2 epitope of the surface antigen (Budkowska et al, 1986). An scFv-formatted version of F124, comprising the same VH and VL sequences, showed dose-dependent binding to, and competitive blocking of IgG and Fab against, r-HBsAg in an ELISA (Passafiume et al, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.
Antibody first published in:
Budkowska et al. Monoclonal antibody recognizing pre-S(2) epitope of hepatitis B virus: characterization of pre-S(2) epitope and anti-pre-S(2) antibody. J Med Virol. 1986 Oct;20(2):111-25. PMID:2430050
Note on publication:
Describes the original generation of this antibody and characterizes its specificity and functionality.