United Kingdom (UK)
Recombinant monoclonal antibody to HCV NS3 Protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Hfab-aNS3.
UniProt Accession Number of Target Protein: P26662
Alternative Name(s) of Target: Genome polyprotein; Core protein p21; Capsid protein C; p21; Core protein p19; Envelope glycoprotein E1; gp32; gp35; Envelope glycoprotein E2; NS1; gp68; gp70; p7; Protease NS2-3; p23; Serine protease NS3; Hepacivirin; NS3P; p70; Non-structural protein 4A
Immunogen: Hfab-aNS3 was isolated from a phage display library obtained from the IgG1/kappa repertoire of an HCV-infected patient who suffered from chronic hepatitis.
Specificity: Hfab-aNS3 binds HCV NS3 protein and is directed against a conformational epitope, as it can bind NS3 in ELISA assays but not Western blots.
Application Notes: ELISA was used to demonstrate that Hfab-aNS3 binds to NS3 protein, with the relative affinity found to be in the range of 1 nmol/L (Prabhu et al, 2004). Through helicase assays it was established that Hfab-aNS3 blocks the helicase activity of NS3 protein (Prabhu et al, 2004). Intracellular expression of Hfab-aNS3 inhibits replication and expression of NS3 protein in a replicon cell line, as shown through immunocytochemistry (Prabhu et al, 2004).
Antibody first published in:
Prabhu et al, 2004. Inhibition of Hepatitis C Virus Nonstructural Protein, Helicase Activity, and Viral Replication by a Recombinant Human Antibody Clone Am J Pathol. 2004 Oct; 165(4): 1163–1173. PMID:15466383
Note on publication:
Describes the isolation of the antibody from a phage display library, and its subsequent characterisation through ELISA and activity assays.