Anti-Hendra and Nipah virus F [1F5]

Description:

Recombinant monoclonal antibody to Hendra and Nipah virus F. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1F5.

Clone Name:

1F5

Published Application(s):

functional assay, neutralize

Published Species Reactivity:

Hendra henipavirus (Hendra virus, HeV), Nipah henipavirus (Nipah virus, NiV)

Product based on this clone:

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Ab03842-1.1 Anti-Hendra and Nipah virus F [1F5]
Hendra henipavirus (Hendra virus, HeV), Nipah henipavirus (Nipah virus, NiV)
Mouse IgG1
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: Q9IH63; O89342

Alternative Name(s) of Target: Fusion glycoprotein; Fusion glycoprotein F0; Protein F; F protein; NiV F; HeV F; NiV; HeV
Immunogen: The original antibody was generated by immunizing mice with recombinant soluble constructs obtained from Nipah or Hendra virus.

Specificity: This antibody binds a pre-fusion conformational epitope on the F glycoprotein of the Nipah and Hendra virus. The Nipah and Hendra virus are spread by bats.

Application Notes: This antibody is reported to broadly neutralize Nipah and Hendra virus by blocking membrane fusion. The cryogenic-electron microscopy (cryo-EM) structures of the antibody in complex with HeV F was determined. Biolayer interferometry was used to characterize the binding of the 1F5 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 1.47 and 1.24 nM for attachment of Fab fragments of 1F5 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG versions of this antibody. The humanized version of the antibody broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml (Dang et al., 2021; PMID:33927387).

Antibody first published in: Dang et al. Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins. Nat Struct Mol Biol. 2021 Apr 29. doi: 10.1038/s41594-021-00584-8. PMID:33927387
Note on publication: Two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion are described.