Anti-HER2 [4D5]

Description:

Recombinant monoclonal antibody to HER2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4D5.

Clone Name:

4D5

Published Application(s):

crystallization, in vivo, inibition, IP, therapeutic, FC

Published Species Reactivity:

Human

Product based on this clone:

{{heading}}

Please Select

Ab01093-1.32 Anti-HER2 [4D5]
Human
Mouse IgG1-D265A
Fc Silenced
Purified
In Stock

UniProt Accession Number of Target Protein: P04626

Alternative Name(s) of Target: CD340; NEU; HER-2; HER-2/neu; ERBB2; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2
Immunogen: The original antibody was generated by immunizing BALB/c mice with NIH 3T3/HER2-3400 cells expressing high levels of p185HER2.

Specificity: The original antibody is directed against the extracellular domain of p185HER2. This antibody does not cross-react with the human EGF receptor (HER1).

Application Notes: The antibody immunoprecipitated p185HER2 from NIH3T3 cells. The binding of the antibody to human tumor cell lines was investigated by fluorescence-activated cell sorting. Increased in cellular fluorescence observed with SK-BR-3 breast adenocarcinoma cells and squamous carcinoma cell line A431, where the binding of the antibody correlated with the levels of p185HER2 expressed by these two cell lines. The antibody inhibited cellular proliferation of SK-BR-3 overexpressing p185HER2 by 56%. The antibody significantly affected the growth of the cell lines MDA-MB-361 and MDA-MB-175-VII, which also express high levels of p185HER2. Further, the antibody completely prevented colony formation by these cells in soft agar. Furthermore, resistance to the cytotoxic effect of tumor necrosis factor alpha is significantly reduced in the presence of this antibody (Hudziak et al., 1989; PMID: 2566907). The humanized IgG1 version of the antibody was constructed and it bound the p185HER2 antigen with higher affinity compared to its parent antibody, it had a similar potency in blocking SK-BR-3 cell proliferation and it supported antibody-dependent cellular cytotoxicity against SK-BR-3 cells (Carter et al., 1992; PMID: 1350088). The structure of the humanized Fab and Fv fragments of the antibody were determined (Eigenbrot et al., 1993; PMID: 8095303). The humanized version of the antibody, trastuzumab, was found to inhibit the growth of human cancer cells and tumor xenografts overexpressing HER2 (Albanell et al., 1999; PMID: 12973420). The Fc silent version of the original antibody was used for mouse tumor growth assays (Campbell et al., 2022; PMID: 35108526). The humanized version of the antibody was used for detection of p185HER-2 expressed on SKBR3 or T6-17 cells by flow cytometry. The antibody was used for immunoprecipitation from T6-17 and B104-1-1 cells (Zhang et al., 1999; PMID: 10493889).

Antibody first published in: Hudziak et al. p185HER2 monoclonal antibody has antiproliferative effects in vitro and sensitizes human breast tumor cells to tumor necrosis factor Mol Cell Biol . 1989 Mar;9(3):1165-7 PMID:2566907
Note on publication: The paper describes the generation and characterization of the antibody, which is specific for HER2.

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience.

Necessary

Always Enabled

Necessary cookies are absolutely essential for the website to function properly. These cookies ensure basic functionalities and security features of the website, anonymously.

Cookie	Duration	Description
ASP.NET_SessionId	session	Issued by Microsoft's ASP.NET Application, this cookie stores session data during a user's website visit.
cookielawinfo-checkbox-advertisement	1 year	Set by the GDPR Cookie Consent plugin, this cookie is used to record the user consent for the cookies in the "Advertisement" category .
cookielawinfo-checkbox-analytics	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checkbox-functional	11 months	The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checkbox-necessary	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-others	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-performance	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
viewed_cookie_policy	11 months	The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.

Analytics

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.

Cookie	Duration	Description
_dc_gtm_UA-49047364-1	1 minute	No description
_ga	2 years	The _ga cookie, installed by Google Analytics, calculates visitor, session and campaign data and also keeps track of site usage for the site's analytics report. The cookie stores information anonymously and assigns a randomly generated number to recognize unique visitors.
_gcl_au	3 months	Provided by Google Tag Manager to experiment advertisement efficiency of websites using their services.
_gid	1 day	Installed by Google Analytics, _gid cookie stores information on how visitors use a website, while also creating an analytics report of the website's performance. Some of the data that are collected include the number of visitors, their source, and the pages they visit anonymously.
ai_session	30 minutes	This is a unique anonymous session identifier cookie set by Microsoft Application Insights software to gather statistical usage and telemetry data for apps built on the Azure cloud platform.
ai_user	1 year	A unique user identifier cookie, set by Microsoft Application Insights software, that enables counting of the number of users accessing the application over time.

Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.

Cookie	Duration	Description
_uetsid	1 day	Bing Ads sets this cookie to engage with a user that has previously visited the website.
_uetvid	1 year 24 days	Bing Ads sets this cookie to engage with a user that has previously visited the website.
MUID	1 year 24 days	Bing sets this cookie to recognize unique web browsers visiting Microsoft sites. This cookie is used for advertising, site analytics, and other operations.
test_cookie	15 minutes	The test_cookie is set by doubleclick.net and is used to determine if the user's browser supports cookies.