Anti-His tag (C-term) [3D5]

Description:

Recombinant monoclonal antibody to His tag (C-term). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3D5.

Clone Name:

3D5

Published Application(s):

WB, ELISA

Published Species Reactivity:

n/a

16

Products based on this clone:

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Please Select

Ab00101-1.1 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG1
Purified
In Stock

Ab00101-10.0 Anti-His tag (C-term) [3D5]
n/a
Human IgG1
Purified
In Stock

Ab00101-11.0 Anti-His tag (C-term) [3D5]
n/a
Human IgG2
Purified
In Stock

Ab00101-12.1 Anti-His tag (C-term) [3D5]
n/a
Human IgG3
Purified
In Stock

Ab00101-13.0 Anti-His tag (C-term) [3D5]
n/a
Human IgG4
Purified
In Stock

Ab00101-2.0 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG2a
Purified
In Stock

Ab00101-22.0 Anti-His tag (C-term) [3D5]
n/a
Hamster (Armenian) IgG
Purified
In Stock

Ab00101-23.0 Anti-His tag (C-term) [3D5]
n/a
Rabbit IgG
Purified
In Stock

Ab00101-3.0 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG2b
Purified
Ships in 4-5 weeks

Ab00101-4.0 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG2c
Purified
In Stock

Ab00101-5.0 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG3
Purified
In Stock

Ab00101-6.1 Anti-His tag (C-term) [3D5]
n/a
Rat IgG1
Purified
In Stock

Ab00101-7.1 Anti-His tag (C-term) [3D5]
n/a
Rat IgG2a
Purified
In Stock

Ab00101-8.1 Anti-His tag (C-term) [3D5]
n/a
Rat IgG2b
Purified
In Stock

Ab00101-9.1 Anti-His tag (C-term) [3D5]
n/a
Rat IgG2c
Purified
In Stock

Ab00101-1.4 Anti-His tag (C-term) [3D5]
n/a
Mouse IgG1
Fc Silent™
Purified
In Stock

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UniProt Accession Number of Target Protein: n/a

Alternative Name(s) of Target: polyhistidine tag
Immunogen: Purified his tagged Fab fragment.

Specificity: This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Antibody first published in: Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. PMID:8994661
Note on publication: Describes the making of the antibody, shows it recognised carboxy-terminal oligo-histidine tags (His tags) of a wide variety of proteins in immuno-assays of cell lines with His tag amplifications.

Binding Curve

Ab00101-1.1 (1 µg/ml, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence. Click on image for more details.

Binding Curve

Ab00101-1.1 (1 µg/ml, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence.

Binding Curve

Western blot using Ab00101-23.0 Click on image for more details.

Binding Curve

Western Blot using Anti-His tag (C-term) antibody 3D5 (Ab00101). His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 3D5 (Ab00101-23.0) at 0.01µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding Curve

IF staining of HEK293 cells using Ab00101-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of HEK293 cells with Anti-His tag (C-term) (Ab00101) 3D5 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 3D5 (Ab00101-23.0) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).