United Kingdom (UK)
Recombinant monoclonal antibody to HLA-A2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BB7.2.
UniProt Accession Number of Target Protein: P04439
Alternative Name(s) of Target: HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28
Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
Specificity: This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
Application Notes: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2a version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complex using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag and EBV peptides using ELISA (PMID:8562505). This antibody was used for screening of human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).
Antibody first published in:
Parham et al. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol. 1981 Dec;3(4):277-99. PMID:7035415
Note on publication:
Describes the generation and purification of this antibody.
Western blot using anti-HLA-A2 antibody BB7.2 (Ab02671). Daudi cells lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (Ab02671-23.0) at 0.3µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 (Ab02671). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BB7.2 (Ab02671-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02671-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western blot using anti-HLA-A2 antibody BB7.2 (Ab02671). Daudi cells lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (Ab02671-23.0) at 0.3µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 (Ab02671). Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (Ab02671-3.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02671-3.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-3.0) followed by staining with Alexa Fluor® 488 secondary antibody.