Anti-HLA-A2/A28 [3PF12]

Description:

Recombinant monoclonal antibody to HLA-A2/A28. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell clone 3PF12.

Clone Name:

3PF12

Published Application(s):

FC, IF

Published Species Reactivity:

Human

Products based on this clone:

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Ab00947-10.6 Anti-HLA-A2/A28 [3PF12]
Human
Human Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

Ab00947-10.0 Anti-HLA-A2/A28 [3PF12]
Human
Human IgG1
Purified
In Stock

Ab00947-10.3 Anti-HLA-A2/A28 [3PF12]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab00947-13.12 Anti-HLA-A2/A28 [3PF12]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

Ab00947-23.0 Anti-HLA-A2/A28 [3PF12]
Human
Rabbit IgG
Purified
In Stock

Alternative Name(s) of Target: Human Leukocyte antigen; HLA-A2; HLA-A28; HLA A2; HLA A28
Immunogen: A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.

Specificity: Amino acid residues 142-145 (TTKH) of the alpha2 domain of the heavy chain of the HLA class I molecules are most likely to be involved in the formation of the epitope recognised by the scfv.

Application Notes: ScFv 3PF12 has been used in immunofluorescence and flow cytometry experiments to test antibody binding to HLA-A2 positive lymphocytes and platelets (Watkins 2000). The scFv has been tested in in vitro assays and have been shown to inhibit target cell killing (Watkins 2003).

Antibody first published in: Watkins et al. The isolation and characterisation of human monoclonal HLA-A2 antibodies from an immune V gene phage display library Tissue Antigens 2000: 55: 219–228 PMID:10777097
Note on publication: Describes the isolation of scFvs against HLA-A2/A28 by V gene phage display.

Flow cytometry analysis of Daudi cells using Ab00947-23.0. Click on image for more details.

Flow cytometry using the Anti-HLA-A2/A28 antibody 3PF12 (Ab00947). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 3PF12 (Ab00947-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.