United Kingdom (UK)
Recombinant monoclonal antibody to HLA-G. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9Y5G6.
UniProt Accession Number of Target Protein: P17693
Alternative Name(s) of Target: HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; HLA-G1sol; HLA-G2sol
Immunogen: The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain amino acid sequence '229SKEGDGGIMSVRSLSED319'.
Specificity: This antibody specifically binds the amino acid sequence 'SKEGDGGIMSVRSLSED' of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor.
Application Notes: This antibody can bind human HLA-G peptide 'KEGDGGIMSVRSLSED' in an ELISA with a binding affinity of Ka= 1.5 X 10^(11) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody can also be used for the immunohistochemical detection of HLA-G5 and HLA-G6 molecules in different gastric cancer tissues using immunohistochemistry (CN113045656).
Antibody first published in: PMID: