United Kingdom (UK)
Recombinant monoclonal antibody to HPi2 . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HIC1-2B4
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Alternative Name(s) of Target: Human Pancreas Endocrine Islet Cell Marker 2; Human pancreatic marker; Endocrine pan-islet cell marker
Immunogen: The original antibody was generated by immunizing BALB/c mice with endocrine cells of the islets of Langerhans.
Specificity: This antibody binds the HPi2 marker on the surface of human pancreatic enriched islet cells.
Application Notes: This antibody binds distinct surface molecules expressed on endocrine cells and have been validated for a number of experimental methods including immunohistochemistry and live cell sorting by flow cytometry. This antibody can be used individually or in combination with others to isolate populations of α, β, exocrine, or duct cells from primary human pancreas by FACS and to characterize the detailed cell composition of human islet preparations (PMID: 19383399, 27641999). This antibody was used in combination with HIC3-2D12 to identify and isolate major subtypes of the human islet cells including α- and β-cells and the purity levels of the isolated cells was determined using immunofluorescence staining (PMID: 31780397). Humanized version of this antibody has also been reported (PMID: 29758224). Chimeric antigen receptor (CAR) with scFv form of this antibody was used in the novel Tregs-based antigen-specific Type 1 Diabetes (T1D) immunotherapy (PMID: 33068914).
Antibody first published in:
Dorell et al. Isolation of major pancreatic cell types and long-term culture-initiating cells using novel human surface markers. Stem Cell Res. (2008); 1(3):183-94. PMID:19383399
Note on publication:
Describes the development of a panel of antibodies for the isolation and study of major cell types of the human pancreas.