Anti-class I MHC [5H7]

Name: Anti-class I MHC [5H7]
SKU: Ab02558
Availability: InStock

Description:

Recombinant monoclonal antibody to class I MHC. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5H7.

Clone Name:

5H7

Published Application(s):

Blocking, IP, FC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02558-3.0 Anti-class I MHC [5H7]
Human
Mouse IgG2b
Purified
Ships in 5-6 weeks

Ab02558-3.3 Anti-class I MHC [5H7]
Human
Mouse IgG2b
Fc Silent™
Purified
Ships in 5-6 weeks

Ab02558-10.0 Anti-class I MHC [5H7]
Human
Human IgG1
Purified
Ships in 5-6 weeks

Ab02558-10.3 Anti-class I MHC [5H7]
Human
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

Ab02558-23.0 Anti-class I MHC [5H7]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P04439

Alternative Name(s) of Target: HLA class I histocompatibility antigen; HLA; Human leukocyte antigen
Immunogen: BALB/c mice were immunized by a series of four to six monthly injections of human PBLs, human Jurkat tumor ceIls, or enriched T lymphocytes.

Specificity: This antibody is specific for HLA class I histocompatibility antigen all different chains. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells.

Application Notes: The biotinylated antibody was used for a blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow cytometry was preformed on human PBMC using this antibody (Woodle et al, 1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are missing a large region of the MHC, including the genes encoding TAP1 and TAP2 (Dangoria et al, 2002; pmid:11978783).

Antibody first published in: Smith et al. Inhibition of T cell activation by a monoclonal antibody reactive against the alpha 3 domain of human MHC class I molecules J Immunol. 1994 Aug 1;153(3):1054-67. PMID:7913109
Note on publication: In this paper the antibody was generated by immunizing mice with human PBLs, human jurkat tumor cells or enriched T lymphocytes