United Kingdom (UK)
Recombinant monoclonal antibody to Human IgG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8E11.
Alternative Name(s) of Target: Human immunoglobulin G
Immunogen: This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species.
Specificity: This antibody reacts with human IgG1, IgG2, IgG3, and IgG4, as well as mangabey, vervet and chimpanzee IgG. It has minimal reactivity with rhesus macaque, cynomolgus monkey or pigtailed macaque IgG.
Application Notes: The ability of this antibody to distinguish between human and macaque IgG has been confirmed in ELISA analysis. As a result of this specificity, this antibody has been used in immunofluorescence analysis to detect intravenously-administered human IgG in formaldehyde-fixed rhesus tissue sections (Schneider et al, 2017).
Antibody first published in:
Schneider et al. Long-term direct visualization of passively transferred fluorophore-conjugated antibodies. J Immunol Methods. 2017 Nov;450:66-72 PMID:28780040
Note on publication:
This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center.