Anti-ICOS [3F12]

Name: Anti-ICOS [3F12]
SKU: Ab01701
Availability: InStock

Description:

Recombinant monoclonal antibody to ICOS. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F12.

Clone Name:

3F12

Published Application(s):

Blocking, ELISA, FC

Published Species Reactivity:

Dog

Products based on this clone:

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Ab01701-201.1 Anti-ICOS [3F12]
Dog
Dog IgG2 (IgG-B)
Purified
In Stock

Ab01701-201.4 Anti-ICOS [3F12]
Dog
Dog IgG2 (IgG-B)
Fc Silent™
Purified
In Stock

Ab01701-202.1 Anti-ICOS [3F12]
Dog
Dog IgG4 (IgG-D)
Purified
In Stock

Ab01701-23.0 Anti-ICOS [3F12]
Dog
Rabbit IgG
Purified
In Stock

Ab01701-2.0 Anti-ICOS [3F12]
Dog
Mouse IgG2a
Purified
In Stock

Ab01701-2.3 Anti-ICOS [3F12]
Dog
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: Q7YR73

Alternative Name(s) of Target: CD278; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; Inducible co-stimulator; Inducible costimulator; caICOS; canine ICOS; dog ICOS
Immunogen: This antibody was raised by immunizing RBF/DnJ mice with caICOS-murine-IgG2a fusion and hybridomas were generated using accepted methods.

Specificity: This antibody specifically recognizes canine ICOS, which enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.

Application Notes: 3F12 can be used to identify canine ICOS and analyse its activity. The antibody is an antagonist of ICOS and exhibits the ability to significantly suppress mixed leukocyte reactions (MLR) (Sato et al., 2013; pmid: 23694952). Anti-ICOS antibody was also shown to be an effective agent that can be used in the treatment development of Canine Chronic Graft-versus-Host Disease (GVHD) (Graves et al., 2017; pmid: 28958896).

Antibody first published in: Sato et al. Inducible Costimulator (ICOS) Up-Regulation on Activated T Cells in Chronic Graft-Versus-Host Disease After Dog Leukocyte Antigen-Nonidentical Hematopoietic Cell Transplantation: A Potential Therapeutic Target Transplantation. 2013 Jul 15;96(1):34-41. doi: 10.1097/TP.0b013e318295c025. PMID:23694952
Note on publication: This article describes the generation and characterization of the antibody 3F12.

Western blot on dog peripheral blood mononuclear cells lysate using Ab01701-23.0. Click on image for more details.

Western Blot using anti-ICOS antibody 3F12 (Ab01701). Dog peripheral blood mononuclear cells lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 3F12 (Ab01701-23.0) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry analysis of dog peripheral blood monocytes using Ab01701-23.0. Click on image for more details.

Flow cytometry using the anti-ICOS antibody 3F12 (Ab01701). Paraformaldehyde fixed dog peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 3F12 (Ab01701-23.0, blue line) at a dilution of 1:100 for 1h at RT.After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of dog peripheral blood monocytes using Ab01701-23.0. Click on image for more details.

Immunofluorescence staining of dog peripheral blood monocytes with anti-ICOS (Ab01701) 3F12 Immunofluorescence analysis of paraformaldehyde fixed dog peripheral blood monocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of 3F12 (Ab01701-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01701-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of dog peripheral blood monocytes using Ab01701-23.0. Click on image for more details.

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