Anti-IgE [D9#3116]

Name: Anti-IgE [D9#3116]
SKU: Ab01391
Availability: InStock

Description:

Recombinant monoclonal antibody to IgE. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D9#3116.

Clone Name:

D9#3116

Published Application(s):

WB, ELISA

Published Species Reactivity:

Dog

Products based on this clone:

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Ab01391-1.1 Anti-IgE [D9#3116]
Dog
Mouse IgG1
Purified
In Stock

Ab01391-41.0 Anti-IgE [D9#3116]
Dog
Dog IgG1
Purified
Ships in 4-5 weeks

Ab01391-23.0 Anti-IgE [D9#3116]
Dog
Rabbit IgG
Purified
In Stock

Ab01391-201.1 Anti-IgE [D9#3116]
Dog
Dog IgG2 (IgG-B)
Purified
Ships in 4-5 weeks

Ab01391-201.4 Anti-IgE [D9#3116]
Dog
Dog IgG2 (IgG-B)
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01391-202.1 Anti-IgE [D9#3116]
Dog
Dog IgG4 (IgG-D)
Purified
Ships in 4-5 weeks

Alternative Name(s) of Target: immunoglobulin E; canine IgE; canine immunoglobulin E; D9
Immunogen: This antibody was raised by immunising BALB/c mice with IgE-rich pooled parasitised dog serum and subsequent creation of hybridomas.

Specificity: The antibody recognises canine IgE (likely C-epsilon 2).

Application Notes: ELISA: Canine IgE-specificity of the D9#3116 antibody was confirmed by ELISA tests with different types of antibodies (DeBoer et al., 1993). WB: This antibody was successfully used in western blot detection of canine IgE in experiments on the dogs' allergies against the hen egg (Shimakura et al., 2016). ELISA: fluorometric ELISA tests were used to assay canine IgE reactivity against different vaccine components in anti-vaccine allergies (Ohmori et al., 2005).

Antibody first published in: DeBoer et al. Production and characterization of mouse monoclonal antibodies directed against canine IgE and IgG. Vet Immunol Immunopathol. 1993 Aug;37(3-4):183-99. PMID:8236798
Note on publication: This paper desribes production and characterisation of the D9#3116 antibody (among other antibodies against canine IgE as well as IgG). Many tests to assess specificity and characterise the antibodies were describes in the article: the ability to cause reverse cutaneous reaction in dog skin, to neutralise Prausnitz-Kustner reaction of atopic dog serum, and binding to different Ig classes in ELISA tests.

ELISA using Ab01391-23.0 Click on image for more details.

ELISA using D9#3116 (Ab01391), Pr00107-20.5, Pr00344-39.5 and Pr00233-1.9. Binding curves of the rabbit IgG chimeric version of the Anti-IgE antibody D9#3116 against Pr00107-20.5 (blue line), Pr00344-39.5 (orange line) andPr00233-1.9 (grey line) to an ELISA plate coated with Pr00107-20.5, Pr00344-39.5 and Pr00233-1.9 at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab01391 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.

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