Anti-IgE [R1E4]

Description:

Recombinant monoclonal antibody to IgE. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R1E4.

Clone Name:

R1E4

Published Application(s):

ELISA, FC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab01115-6.1 Anti-IgE [R1E4]
Mouse
Rat IgG1
Purified
Ships in 4-5 weeks

Ab01115-6.4 Anti-IgE [R1E4]
Mouse
Rat IgG1
Fc Silent™
Purified
In Stock

Ab01115-2.0 Anti-IgE [R1E4]
Mouse
Mouse IgG2a
Purified
In Stock

Ab01115-2.3 Anti-IgE [R1E4]
Mouse
Mouse IgG2a
Fc Silent™
Purified
In Stock

Ab01115-1.32 Anti-IgE [R1E4]
Mouse
Mouse IgG1-D265A
Fc Silenced
Purified
Ships in 4-5 weeks

Ab01115-23.0 Anti-IgE [R1E4]
Mouse
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: P06336

Alternative Name(s) of Target: Immunoglobulin E
Immunogen: This antibody was generated in rat against murine IgE in adjuvant.

Specificity: This antibody is specific for murine IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

Application Notes: In the original study, this antibody and other clones (C12B9, 23G3, and B1E3) of the monoclonal anti-mIgE antibodies were used in conjunction with the recombinant chimeric mIgE-human IgG1 molecules (Keegan et al., 1991). It has been demonstrated that the Cϵ3 domain is important in the binding of mIgE to the murine B cell FcϵRII as well as to the murine mast cell FcϵRI (Keegan et al., 1991). Also, while the Cϵ4 domain had no effect on binding to the FcϵRI, the presence of the Cϵ4 domain influenced the binding of the recombinant IgE to the FcϵRII (Keegan et al., 1991). This antibody blocks IgE binding to mouse or rat FceRI and can be used in mouse IgE ELISA. In addition, this antibody could be used, for instance, to develop a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses (Manetz et al, 1999), or to study the kinetics of the ligand-IgE interaction (Posner et al., 1992).

Antibody first published in: Keegan et al. Characterization of new rat anti-mouse IgE monoclonals and their use along with chimeric IgE to further define the site that interacts with Fc epsilon RII and Fc epsilon RI. Mol Immunol. 1991 Oct;28(10):1149-54. PMID:1717839
Note on publication: Describes the original generation of this antibody and its subsequent characterisation to further define the site that interacts with Fc epsilon RII and Fc epsilon RI.

ELISA using Ab01115-23.0 Click on image for more details.

ELISA using R1E4 (Ab01115), Pr00107-20.5, Pr00344-39.5 and Pr00233-1.9. Binding curves of the rabbit IgG chimeric version of the Anti-IgE antibody R1E4 against Pr00107-20.5 (blue line), Pr00344-39.5 (orange line) andPr00233-1.9 (grey line) to an ELISA plate coated with Pr00107-20.5, Pr00344-39.5 and Pr00233-1.9 at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab01115 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.

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