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- Ab00856-23.0 Anti-IgG3 [NH3/15.8]
- Human
- Rabbit IgG
- Purified
- In Stock
- Ab00856-1.1 Anti-IgG3 [NH3/15.8]
- Human
- Mouse IgG1
- Purified
- In Stock
Recombinant monoclonal antibody to IgG3. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NH3/15.8.
Alternative Name(s) of Target: IgG1/3; immunoglobulin G1; human immunoglobulin; immunoglobulin G3
Immunogen: This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
Specificity: This antibody recognizes human IgG3.
Application Notes: NH3/15.8 antibody was used to successfully detect IgG3 antibodies.
Antibody first published in: The use of monoclonal antibodies to human IgG in blood transfusion serology. Biotest Bulletin 4: 348-352 (1983) PMID: Note on publication: This article describes the generation and characterisation of the NH3/15.8 antibody.
ELISA using NH3/15.8 (Ab00856) with different antigens. Binding curves of the mouse IgG1 chimeric version of the Anti-IgG3 antibody NH3/15.8 (Ab00856-1.1) to an ELISA plate coated with Mouse IgG1 Anti-Fluorescein [4-4-20 (enhanced)] (Ab00102-1.1, blue line), Human IgG1 Anti-Fluorescein [4-4-20 (enhanced)] (Ab00102-10.0, orange line), Human IgG1 Anti-hapten 4-hydroxy-3-nitrophenyl acetyl (NP) [B1-8] (Ab00104-10.17, gray line) and cAb2780-10.64 (yellow line) at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 1000 to 0.45 ng/ml was performed using Ab00856 antibody.